BIOSYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES OF EUPHORBIA MILII DES MOUL. LEAF EXTRACT

Abstract

Synthesis of nanomaterials may involve various routes including physical, chemical and biological approaches. The biological green synthesis of nano particles is gaining attention due to its cost effective, ecofriendly and possibility for easy large scale production. In this present study, leaf extract of Euphorbia milii was used to synthesize silver nano particles and aimed to study the antibacterial activity of the synthesized silver nanoparticles. The silver nano particles synthesized were confirmed by their change of colour to dark brown due to the phenomenon of surface plasmon resonance. The rapid reduction of silver (Ag+) ions was monitored by using UV-visible spectrophotometer. Transmission electron microscopy (TEM) and Scanning electron Microscopy (SEM) showed that the synthesized nano particles are varied from 20-50 nm with spherical shape. FTIR analysis showed the functional groups involved in the AgNps formation. Biological evaluation of silver nanoparticles was also done against Gram positive and Gram negative bacteria for their future applications in biomedicine. The synthesized silver nanoparticles exhibited good antibacterial activity against both Gram-negative and Gram-positive bacteria tested. This work proved the potential antibacterial activity of silver nano particles synthesized from leaves of E.milii.