ISOLATION AND CHARACTERIZATION OF LACTOBACILLI FROM CAECUM OF RAT FED WITH FRUCTOOLIGOSACCHARIDES

Abstract

Aim: Administration of fructooligosaccharide supports the growth of beneficial bacteria. This study aims to isolate and characterize Lactobacilli from caecum of rat fed with FOS. Materials and Methods: Lactobacilli were isolated from the caecum of the rat fed with fructooligosaccharide for one month. Isolated Lactobacilli were characterized to study the probiotic potential and effectiveness of antimicrobial activity against S. aureus and E. coli, common pathogens of large intestine. Agar well diffusion method was used to study the antimicrobial activity. Growth conditions (temperature, pH and incubation conditions) for isolate were optimized using full 3³ factorial design. Results and Discussion: The isolates were found to be facultative anaerobes, Gram positive, non-spore forming and non motile forming either bacilli or coccobacilli. Optimization study revealed when isolates grown at 37^oC, pH 7 and anaerobic conditions, maximum growth was obtained. Conclusion: Isolated Lactobacilli showed acid and bile resistance. Bacteriocin produced by the isolated Lactobacilli (RCL3) from rat caecum showed antimicrobial activity against S. aureus and E. coli.