FORMULATION AND EVALUATION OF NANO-EMULSION GEL OF AMPHOTERICIN B FOR TREATMENT OF SKIN INFECTIONS

Abstract

Amphotericin B is a polyene antifungal agent with activity in vitro against a wide variety of fungal pathogens. Amphotericin B exerts its antifungal effect by disruption of fungal cell wall synthesis because of its ability to bind to sterols, primarily Ergosterol, which leads to the formation of pores that allow leakage of cellular components. This affinity may also account for its toxic effects against select mammalian cells. Amphotericin B is generally considered cidal against susceptible fungi at clinically relevant concentrations. The gel was formulated by changing the polymer ratio. FT-IR study confirmed the purity of drug and revealed no interaction between the drug and excipients. Gel formulations were characterized for drug content, pH determination, drug content, in-vitro drug release and particle size. Among the nine formulations, F9 was selected as the best formulation as its %CDR after 08 hours was 93.92%. The drug content of the F9 formulation was 98.12%. Gel formulation F9 was found to be stable at 30 ±2°C and 65 ± 5 RH. It was found that at 40 ± 2°C and 75 ± 5 RH the gel formulation was not stable and %CDR was decreased.