EVALUATION OF ETHANOLIC EXTRACT OF HEMIDESMUS INDICUS IN COMBINATION WITH OCIMUM BASILICUM FOR DEXTRAN SULFATE SODIUM INDUCED COLITIS IN MICE

Abstract

Inflammatory bowel disease (IBD) is a chronic intestinal disease which mainly involves Ulcerative colitis and Crohn’s disease. Our study shows mostly the Dextran Sodium Sulphate induced colitis model. Ulcerative colitis is the long-lasting inflammation that is primarily caused due to immune dysregulation, microbiota and other genetical and environmental factors. Dextran sodium sulphate induces the colitis by damaging the intestinal epithelial barrier. In the present study, Hemidismus indicus and Ocimum basilicum ethanolic extracts showed the anti-inflammatory activity at a dose 250-500 mg/kg and compared with standard drug sulfasalazine. Among VI groups, group-I animals served with saline, group –II animals are given only Dextran Sodium Sulphate, other groups along with Dextran sodium sulphate they receive ethanolic extracts at different doses according to body weights, the study was conducted at 16 days at the end of the day the animals were sacrificed by giving ether, colon lengths and weights were measured, for group –II animals there is decrease in colon length and weight and also shows the rectal bleeding and slight lesions. Whereas other groups don’t show any sores and rectal bleeding. Based on this we confirm the anti-inflammatory activity. After identification of Group – II animals lesions we understood chemoprotective effect against Dextran Sodium Sulphate induced ulcerative colitis.