SIMULTANEOUS DETERMINATION OF METFORMIN AND GLIBENCLAMIDE BY ULTRA-VIOLET SPECTROPHOTOMETRY

Abstract

This research deals with simultaneous determination of Glibenclamide and Metformin Hydrochloride in combined dosage form. The method utilized simultaneous equation method for analysis using 0.1 N HCL as a solvent. The two wavelengths 300 nm and 233 nm were selected for determination of Glibenclamide and Metformin Hydrochloride respectively. Beer^’s law was obeyed in concentration range of 10-60µg/ml and 2-12µg/ml for Glibenclamide and Metformin Hydrochloride respectively. The recovery studies confirms the accuracy of the projected method and the results were validated as per ICH guidelines. The proposed method is simple and rapid, can be employed for determination of Metformin Hydrochloride and Glibenclamide in pharmaceutical dosage form.