PROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF HORDEUM VULGARE SEED ON GENTAMICIN INDUCED NEPHROTOXICITY

Abstract

Objective of the study was to evaluate protective effect the Ethanolic extract of seeds of Hordeum vulgare was tested in an animal model of
nephrotoxicity. Nephrotoxicity was induced by the addition of Gentamicin (80 mg/kg; i.p) to the normal diet of Wistar albino rats for a period of 10
days. Group I served as a normal control. Group II served as nephrotoxicity control. Group III, IV and V were treated with ethanolic extract of
Hordeum vulgare (EHV) at 100, 250 and 500 mg/kg respectively. The effects of EHV on various biochemical parameters were studied in
Nephrotoxicity rats. Gentamycin induced nephrotoxicity in rats, there were significant elevated kidney weight, body weight loss and blood vessel
congestion and scattered mononuclear inflammatory cell within the interstitial in gentamycin induced rats. In vivo antioxidant parameters including
protein, malondialdehyde, superoxide dismutase, reduced glutathione and catalase (CAT) were also determined. EHV significantly maintained the
renal parameters like serum urea, BUN, creatinine. The extract also induced a significant decrease in MDA which increased in nephrotoxicity control
rats. The extract also significantly increased reduced glutathione, superoxide (100,250) and CAT in nephrotoxicity rats which were markedly
decreased in gentamicin induced nephrotoxicity in rats. This study demonstrates the protective activity of Hordeum vulgare seeds and rationalizes
their medicinal use for the treatment of nephrotoxicity.