CHARACTERIZATION OF SECONDARY METABOLITES WITH MOSQUITO-LARVICIDAL ACTIVITY EXTRACTED FROM ACTINOMYCETES ISOLATED FROM DIFFERENT ECOLOGICAL NICHE

Abstract

As mosquito vector has major role in transmission of mosquito borne diseases like malaria, filariasis, dengue etc, mosquito control program has prime importance to restrict mosquito development. The Actinomycetes secondary metabolite possessing mosquito-larvicidal activity can be part of this control program. Actinomycetes are common habitat of soil. During life cycle, it produces various secondary metabolites like antibiotics, growth promoter substances, insecticides. The insecticidal nature of secondary metabolites can be used to kill mosquito larvae. In current study 107 soil samples were collected from different ecological niche like farm area, forest area, open ground area and polluted area of west Maharashtra, India. All these samples were processed by microbiological methods to obtained 197 actinomycetes isolates. In primary screening 16 isolates was found to be possessing mosquito-larvicidal activity. LC50 study of secondary metabolite produced by 16 isolates helps in selecting two potent producers. The functional groups of ethyl acetate extracted secondary metabolite were analyzed by FTIR. These two effective actinomycetes isolates were identified by 16S-rRNA technique.