ANTIFUNGAL POTENTIAL AGAINST Aspergillus flavus: SECONDARY METABOLITE COMPOUND FROM UNIQUE ORGANISM OF LICHEN Teloschistes flavicans

Abstract

Lichen is a unique symbiotic organism formed from the mutualism relationship between fungus and algae. The aims of this research are to isolate and to examine antifungal activity of secondary metabolite compound from lichen Teloschistes flavicans. This research was conducted by extracting lichen dried powder using acetone solvent for 3×24 hours. The extract was isolated by Column Chromatography (CC) and Thin Layer chromatography (TLC) with G60 silica gel as stationary phase. Then it was eluted by n-hexane and ethyl acetate solvents in a gradient elution. The isolate was obtained and purified by recrystallization process then determined by using UV-VIS, FTIR, LC-MS, and 1H-NMR. The isolated compound is an orange needle "3- [1’-(2”,3”-dihydroxy-phenyl)-propyl]-7hydroxy-chroman-4-one" with the formula of C18H18O5. The antifungal activity test against Aspergillus flavus using disc diffusion method showed that the inhibitory zone was 11 mm in the concentration of 1000 mg mL^-1.