SCREENING OF Vitis vinifera FOR FLAVONOID CONTENT AND FREE RADICAL SCAVENGING POTENTIAL

Abstract

V. vinifera is one of the important fruit crop, characterized by substantial number of soluble flavonoids including quercetin, the clinically proved
therapeutic agent. Present study has been focused on analysis of flavonoids together with quercetin in aerial parts of V.vinifera and their correlation
with radical scavenging activity (RSA). Two varieties of black cultivars viz. Sharad and Sonaka seedless were selected. Pr esence of flavonoid was
confirmed by phytochemical analysis. Bands of flavonoids were detected by Preparative thin layer chromatography (PTLC). Total flavonoid content
(TFC) and radical scavenging activity (RSA) was estimated by AlCl3 colorimetric assay and DPPH assay respectively. Quantification of quercetin
was done by HPLC. Findings of analysis showed high abundance of flavonoids in almost all parts of both cultivars. Rf value for quercetin was found
to be 0.85. Aerial parts of both cultivars showed significant amount of TFC. Petiole of Sonaka seedless showed highest content of flavonoid (0.28 ±
0.06 mg/g QE). Whereas, stem of Sharad seedless showed less content (0.10 ± 0.02 mg/g QE). Concentration of quercetin in berry skin of Sharad
seedless was found to be 2133.4 ppm. Order of RSA for both the cultivars was Petiole>Stem>Leaf lamina. In Sharad seedless less significant
correlation was found between TFC and RSA. Whereas positive correlation was shown by Sonaka seedless Therefore, it can be concluded that in
addition to skin, almost all the aerial parts of V.vinifera are rich source of flavonoids with noteworthy RSA i.e antioxidant potential. Thus, significant
amount of flavonoids can be efficiently extracted from these aerial parts of V.vinifera which can be used for healing of various diseases and disorders