ANALYTICAL METHODS DEVELOPMENT & VALIDATION FOR SIMULTANEOUS ESTIMATION OF LOPINAVIR & RITONAVIR IN PHARMACEUTICAL FORMULATION BY SIMULTANEOUS EQUATION METHOD USING UV SPECTROPHOTOMETRY

Abstract

The present work deals with the simultaneous estimation of Lopinavir and Ritonavir in bulk and pharmaceutical dosage form using the UV method. Shimadzu UV system was used for analysis. The solvent selected for analysis was acetonitrile: water (30:70v/v). The wavelength of Lopinavir and Ritonavir were found 257.5 nm and 240.0 nm respectively. The Linearity for simultaneous equation method was studied by plotting a graph Conc. Vs. absorbance, Linearity was observed in the concentration range 80-180µg/ml for Lopinavir and 10-60 µg/ml of Ritonavir Coefficient of correlation (R²) was found to be 0.997 and 0.998 for Lopinavir and Ritonavir respectively. The LOD was found to be 2.379µg/ml and 0.66 µg/ml for Lopinavir and Ritonavir respectively. LOQ was found to be 7.21µg/ml and 2 µg/ml for Lopinavir and Ritonavir respectively. The developed method was employed for the analysis of marketing formulation. The amount of drug obtained was in accordance with label claim. The recovery studies were carried out at three levels, i.e.80 %, 100% and 120% by the standard addition method. The precision of the proposed method was also established. The method was found to be accurate and precise.