ANTIDIABETIC POTENTIAL OF POLY HERBAL FORMULATION ON ALLOXAN INDUCED DIABETIC RATS

Abstract

Aim: The present study was aimed to design the effect of poly herbal formulation against alloxan induced diabetic rats. The formulation was prepared by using three common herbal plants namely Alternanthera sessilis Linn., Amaranthus viridis Linn., and Boerhavia diffusa Linn., and glibenclamide as a standard. Methods: The rats were divided in to five groups each group comprising of five rats. The animals were grouped as: Group I-Normal control, Group II- Alloxan induced (150mg/kg b.wt), Group III- Alloxan + Poly Herbal Formulation (200mg/kg b.wt0, Group IV- Alloxan + Poly Herbal Formulation (400mg/kg b.wt) and Group V-glibenclamide (1mg/kg b.wt) for 30days. After the experimental period of 30 days, the blood and tissue samples were collected for preclinical trials. The parameter studied were fasting blood glucose, serum insulin, glucokinase, glucose -6- phosphatase, total protein, hepatic glycogen, serum cholesterol, triglycerides, serum urea and creatinine, lipid peroxide and superoxide dismutase. Results: Alloxan induced diabetic rats showed significant increase in fasting blood glucose, glucose -6- phosphatase, serum urea and creatinine, serum cholesterol and triglycerides and lipid peroxide and it also decrease in serum insulin, glucokinase, serum protein, hepatic glycogen and superoxide dismutase. Oral administration of Poly herbal formulation restored the levels of biochemical parameters and the level of glucose metabolizing enzymes. Conclusion: From the above findings, suggested that the poly herbal formulation possess antidiabetic activity and it can be used as starting point for the development of herbal based novel drug against diabetes.