ANTIOXIDANT AND ANTI-INFLAMMATORY EFFECTS OF PHASEOLUS VULGARIS L. SEEDS ETHANOL EXTRACT: AN IN-VITRO STUDY

Abstract

Aim: The aim of the present study was to find the antioxidant and anti-inflammatory potential of Phaseolus vulgaris L. seeds ethanol extract using in- vitro models. Method: The antioxidants effects of Phaseolus vulgaris L. seeds ethanol extract was determined using free radical scavenging assay, reducing power assay, hydrogen peroxide radical scavenging and metal chelating activity. Inhibition of protein denaturation, anti-proteinase action and membrane stabilization methods which includes heat induced haemolysis, hypotonicity-induced haemolysis and anti-lipoxygenase activity were used to resolve the power of anti-inflammatory agents. IC50 values are calculated by linear regression method. Different concentrations (100 - 500 µg/ml) and (20 - 100 µg/ml) of P.vulgaris ethanol extract and reference standards respectively, were used to evaluate the antioxidant and anti-inflammatory activities. Results: The result suggests that the Phaseolus vulgaris L. seeds ethanol extract possesses antioxidant and anti-inflammatory activities when compared with standard drugs. Conclusion: From the study it is concluded that various phytoconstituents flavonoids and other related polyphenols present in Phaseolus vulgaris L. seeds ethanol extract are responsible for the antioxidant and anti-inflammatory activities.