RADICAL SCAVENGING ACTIVITY OF NEOLAMARCKIA CADAMBA (ROXB) BOSSER IN NEPHROLITHIASIS RELATED OXIDATIVE STRESS

Abstract

Objective: To investigate the role of Neolamarckia cadamba (Roxb) Bosser in reducing nephrolithiatic oxidative stress in wistar rats. Methods: Nephrolithiasis induced in rats by supplementing ethylene glycol (EG) in drinking water for 28 days. Animals were divided into six groups, each containing six viz. vehicle control, nephrolithiatic control and cystone (750mg/kg, p.o) served as antilithiatic control. Methanol fruit extract of N. cadamba fruit extract (MFNC) in doses of 200, 400 mg/kg, p.o. were administered to rats after receiving EG in both curative and preventive regimes. DPPH activity was assessed to evaluate the antioxidant activity of the extract. Oxidative stress reducing property of MFNC in nephrolithiasis was estimated for both enzymatic and non enzymatic markers of oxidative stress like Thiobarbaturic acid relative substances (TBARs), conjugated diene (CD), Lipid hydroperoxides (HP), reduced glutathione (GSH), SGOT and SGPT. Estimation of Antioxidant enzymes, Superoxide dismutase (SOD), Catalase (CAT) and Glutathione Reductase (GST) were evaluated. Results: Lithiatic groups elevated the level of oxidative stress markers like TBARs, CD, HP, SGOT, SGPT whereas the MFNC extract lowered these values towards control. Antioxidant enzymes (SOD, CAT and GST) concentrations elevated by the MFNC administration by lowering the oxidative stress. Conclusion: the results suggest the renoprotection of MFNC by preventing the oxidative stress induced by nephrolithiasis.