IN VITRO ANTIOXIDANT ACTIVITY OF ANTIBACTERIAL METABOLITES FROM STREPTOMYCES SPECIES KSRO-04

Abstract

Antioxidants have gained importance as pharmacological agents and have been consumed in various forms mainly as fruits, vegetables and dietary supplements. These antioxidants are majorly obtained from plant source or have been synthesized chemically. This study concentrated on the in vitro evaluation of antioxidant property of a water soluble antibacterial metabolite from Streptomyces species KSRO-04. The antioxidant activity was evaluated by Total phenolic content, total flavonoid content, Total antioxidant activity, DPPH assay, Lipid peroxidation inhibition assay, Nitric oxide scavenging activity, Super oxide anion scavenging activity, Metal ion chelating activity and total reductive capability. The metabolite was characterised using TLC and purity was assessed by HPLC. The UV absorption indicated absorption maximum of 360 nm suggesting a polyene nature of the metabolite. The metabolites showed a moderate antioxidant activity with good nitric oxide scavenging activity and also hinted that the anti oxidant activity is not solely due to the phenolic compounds. The metabolite from the Streptomyces species KSRO-04 is potent both as antimicrobial agent and an antioxidant agent which suggest its dual and beneficiary role as a pharmacological agent.