BACTERIAL CHARACTERIZATION OF SILVER NANOPARTICLES FROM TEMBAGAPURA SOIL SAMPLE ISOLATE, PAPUA, INDONESIA

Abstract

Characterization of silver nanoparticle-producing bacteria from Tembagapura, Papua, Indonesia soil samples isolates has been investigated. Bacteria characterization was carried out macroscopically, microscopically, biochemically and molecularly. Furthermore, the results of the formed silver nanoparticles were characterized using UV-VIS spectroscopy, Fourier transform infrared (FTIR) and scanning electron microscopy (SEM). The experimental results showed maximum absorbance at 414 nm in TP10-1 isolates in UV-Vis spectroscopy. FTIR spectra of silver nanoparticles samples of TP10-1 isolates showed strong peaks in wave numbers 1637.65 cm-1 and 3329.47 cm-1. SEM micrographs reveal the formation of well dispersed silver nanoparticles. Silver nanoparticles of TP10-1 isolates that was measured by the imageJ program had an average particle size of 16,991 nm. Bacterial isolates with TP10-1 sample code which are identical to the Bacillus cereus strain GCF1I2 was able to synthesize silver nanoparticles.