ACTIVITY OF ETHYL ACETATE FRACTION OF CELERY HERB (Apium graveolens L.) ON CREATININE AND UREA LEVEL IN ETHYLENE GLYCOL INDUCED RATS

Abstract

The utilization of celery by the community in Indonesia was known as a seasoning, particularly in soups to enrich the taste of food, the potential effect of celery herb especially as herbal medicine both in preventive or curative therapy are not widely known yet. The celery herb is rich in flavonoids content which has potential effect to dissolve kidney stones.The purpose of this study was to determine the effect of ethyl acetate (Apium graveolens L) fraction on urea and creatinine levels in male wistar rats induced by ethylene glycol. The in vivo test was conducted on male Wistar rats that divided into 6 groups. Group I as a normal control without treatment, Group II as a negative control induced by ethylene glycol, Group III as a positive control was given Batugin Elixir, Group IV, V and VI were given ethyl acetate fraction of celery at 50, 100 and 150 mg / kgbw respectively . Blood sample was collected through cardiac puncture method. The results showed that ethyl acetate fraction of celery herb had an effective activity at a dose of 150 mg / kgbw which was significantly different to the negative control group (P <0.05) measured by a reduction of urea and creatinine level on male wistar rats induced by ethylene glycol.