TOXICITY OF COPPER NANOPARTICLE ON HAEMATOLOGY AND BIOCHEMISTRY OF FISH, TILAPIA MOSSAMBICA

Abstract

Present investigation was carried out to study the toxicity of copper nanoparticle on haematology (blood) and biochemistry in different organs (gills, liver and muscle) of fish, Tilapia mossambica. Fishes were exposed to different levels of copper nanoparticle for a period of 14 days and control was also maintained simultaneously. Blood samples were collected from experimental and control animals and haematological study was carried out. The results of the haematological study showed that there was a decrease in haematological parameters (total WBC count, basophils, eosinophil, neutrophils, lymphocytes, monocytes, platelet count, total RBC count, haemoglobin, haematocrit, MCV, MCH and MCHC) compared to that of control. Since major biochemical constituents such as carbohydrates and proteins of the body play an important role in body’s construction and energy metabolism, the work was further extended to study biochemical estimation in different organs (gills, liver and muscle) of Tilapia mossambica after exposure to different levels of copper nanoparticle for 14 days. The results of the biochemical estimation indicated that the carbohydrates and proteins were highly reduced in the gills followed by the liver and muscle and carbohydrate was much depleted than protein when compared to control.