IN VITRO ANTIOXIDANT AND ANTI- INFLAMMATORY OF THE FLOWER EXTRACTS OF HIBISCUS SABDARIFFA L.

Abstract

Objective : To evaluate the antioxidant and anti-inflammatory activities of the solid powder obtained from the ethyl acetate fraction from the flower Hibiscus sabdariffa L. Methods: The flower extract was evaluated for antioxidant activity by 1,1–diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay and reducing power assay was carried out by FRC (Ferric Reducing Capacity) assay method. The in vitro anti-inflammatory activity was evaluated using human peripheral blood mononuclear cells (PBMC) were stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO) production method. Results : The solid powder obtained from the ethyl acetate fraction from the flower Hibiscus sabdariffa L showed a good antioxidant activity in scavenging DPPH radical and FRC assay with compared standard sample. This solid powder also showed good anti-inflammatory activity in cell viability (LPS induced PBMCs) assay and nitric oxide (NO) assay. Conclusion : These results suggest that the solid powder obtained from the ethyl acetate fraction from the flower Hibiscus sabdariffa L have significant antioxidant and anti-inflammatory activities.