ISOLATION IPG3-1 AND IPG3-3, ENDOPHYTIC FUNGI FROM DELIMA (PUNICA GRANATUM LINN.) TWIGS AND IN VITRO ASSESSMENT OF THEIR ANTI MICROBIAL ACTIVITY

Abstract

IPG3-1,2,3,4,5 were endophytic fungi isolated from Delima twigs via direct seed inoculation on Potato Dextrose Agar (PDA). Macroscopic identification of the isolates showed clearly that IPG3-3 and 4 have radial wrinkles in the centre with black spots while IPG3-5 has flower shape in the middle. The microscopic observation on the other hand, showed that IPG3-1 and 3 has branched septate while IPG3-4 has unbranched septate. Furthermore, IPG3-2 and 5 were branched but septateless. Overall, these endophytes showed no spore formation. Secondary metabolites of IPG3-1 and IPG3-3 endophytic fungi isolates demonstrated stronger inhibition zone percentage against Candida albicans and than towards Staphylococcus aureus and Escherichia coli when campare to their respectively positive control amphotericin B and Chloramphenicol.