PHYTOCHEMICALS ANALYSIS AND TLC FINGERPRINTING OF METHANOLIC EXTRACTS OF THREE MEDICINAL PLANTS

Abstract

The present work is done on three medicinal plants (Enhydra fluctuans, Lecuas aspera and Dillinia indica) in order to investigate the presence of the various types of Phytoconstituents. The leaves of all three plants were extracted using methanol as solvents. For the purpose of phytochemical investigation, Preliminary qualitative chemical test and TLC were mainly used. Thin layer chromatography (TLC) has been carried out on all the three plants in two different solvent systems, which showed different Rf value. TLC profiling of all these three extracts confirm about the presence of various phytochemicals. Different Rf (Retention factor) value of various phytochemicals provide valuable clue regarding their polarity and selection of solvents for separation of phytochemicals.