ANTINOCICEPTIVE AND ANTI-INFLAMMATORY ACTIVITIES OF CROTON SPARSIFLORUS MORONG LEAVES IN ANIMAL MODELS

Abstract

A depth study was carried out to find out the potential of chloroform extract of Croton (C) sparsiflorus morong. (Family-Euphorbiaceae) leaves on antinociceptive, behavioral study and anti-inflammatory effects using various animal models The dried, powdered leaves of, C. Sparsiflorus Morong were extracted successively with petroleum ether (60-80°C)and chloroform in soxhlet apparatus.The chloroform extract (yield 5.60% w/w with respected to dry powdered plant material) was selected for all experimental procedure. Two models were put to investigate the effects of nociception, by the tail immersion and hot plate method on Swiss albino mice and anti inflammatory effect were studied by employing the carrageenan induced rat paw edema test in. adult Wister albino rats. Behavioral activity was carried out by elevated plus maze method in Swiss albino mice. Results shows that the CSCE has significant antinociceptive effect (P < 0.001) at the dose levels of 100, 200 and 400 mg/kg, orally in mice and also produced remarkable anti-inflammatory effect (P < 0.001) at the same dose levels used in the rats. Behavioral study of the CSCE has no differential anxiolysis effect when used orally. It concludes that, CSCE possessed remarkable antinociceptive effect and anti-inflammatory effect but no anxiolytic effect on animal models.