GAS CHROMATOGRAPHY TROUBLESHOOTING STRATEGY FOR ANALYTICAL CHEMISTRY

Abstract

The most utilized of all the chromatographic techniques is gas chromatography. This technique gives good precision and accuracy. Essentially a gas chromatography separates of a gaseous mixture in a column and detects the components as they are eluted. The response of the detector is plotted as a function of time & carrier gas volume on a strip chart recorder. Gas chromatography is one of the sole forms of chromatography that does not utilize the mobile phase for interacting with the analyte. It can be configured for analysis of specific compounds using a wide choice of options. GC is a very well- established technique for determination of residual solvents, non chromophoric impurities in drug substance, drug product and environmental contaminant identification. Troubleshooting GC instrumentation and separation require a fundamental understanding of how the instrument functions and how the separation works. This article helps the reader to understand the relationship between the problems and remedy. The objective of this work is it serves as both troubleshooting guide and a GC learning tool.