ASSESSMENT OF ANTICANCER ACTIVITY OF ETHANOLIC EXTRACT OF BORASSUS FLABELLIFER LINN. LEAF AGAINST EHRLICH ASCITES CARCINOMA (EAC) AND DALTONS ASCITES LYMPHOMA (DAL) IN SWISS ALBINO MICE

Abstract

The medicinal plants have very important role in the health of human being as well as animals. The present study was thus designed to investigate the anticancer activity of ethanolic leaf extract of Borassus flabellifer Linn. And evaluated by in-vitro and in-vivo experimental models. To achieve objectives, the plant was subjected to phytochemical screening and tested for oral toxicity. The in-vitro study was carried out by means of MTT assay and Trypan blue dye exclusion assay using EAC cell line and DAL cell lines. The in-vivo anticancer activity was evaluated against EAC and DAL tumor bearing mice by liquid tumor models. Preliminary phytochemical screening confirmed the presence of flavanoids, glycosides, Tannins, Phenolic, Steroids and triterpenoids etc. The plant showed good cytotoxic effect on EAC cell line and DAL cell line in both MTT assay and Trypan blue dye exclusion assay. Oral administration of ELBF in tumor bearing mice for 14 days, showed significant reduction in the percent increase in body weight, tumor volume, tumor weight, viable cell count and increased non-viable cell count when compared to the untreated mice of the EAC control group and DAL control group. The restoration of the haematological parameters towards the normal control was also observed. The results suggested that the Borassus flabellifer exhibits significant anticancer activity towards both methods.