THE ASSOCIATION OF RESISTIN GENE –420C/G (RS1862513) SINGLE NUCLEOTIDE POLYMORPHISM WITH TYPE TWO DIABETES MELLITUS IN IRAQI POPULATION

Abstract

Background: Worldwide studies have shown polymorphisms within the resistin gene like–420C/G (rs1862513) to verify the effect of these polymorphisms in the occurrence of T2DM. Aim: To study the association of –420C/G with T2DM in Iraqi population. Methods: The study consisted of 400 T2DM patients and 400 healthy control individuals. Genotyping of –420C/G polymorphism is achieved by PCR–RFLP. DNA was extracted from whole blood and genotyping was carried out by specific primers to amplify fragments for digestion with restriction enzyme. Bbs I restriction enzyme used for digestion followed by electrophoresis on agarose gel. Various statistical analyses were used to analyze the data. Results: The genotype and allele frequencies of rs1862513 SNP revealed insignificant differences among the homo (GG) (OR= 1.42 ,CI 95%;0.95-2.11;P=0.08), hetero (CG) (OR= 1.07 CI 95%; 0.78-1.45, P= 0.7), dominant (OR = 1.42, CI 95%; 0.96-2.11, P= 0.08) and recessive (OR = 1.37, CI 95%; 0.96-

1.98, P= 0.08) models. Furthermore, the minor allele frequency (G) was insignificantly increased (P= 0.21) in T2DM when compared with the control group. Conclusion: Resistin gene (rs1862513) polymorphism was exhibited non-significant association with T2DM in Iraqi population.