COMPARATIVE STUDY OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ULTRAVIOLET SPECTROPHOTOMETRY METHODS ON DIFFERENT BRANDS OF METRONIDAZOLE TABLETS MARKETED IN MAIDUGURI METROPOLITAN COUNCIL OF BORNO STATE

Abstract

The active ingredient of ten brands of Metronidazole tablets were determined using High Performance Liquid Chromatography and Ultra Violet Spectrophotometry to determine which of the assay methods is better and if the drug is of the required standards. For the ultra violet spectrophotometry samples A,B,C,G and I have the required amount specified while sample D,E,F,H and J failed the required specifications. The High Performance Liquid Chromatography assay showed that samples A,B,C F,G and H are of the required specifications while D,E,I and J failed the required specifications. Samples A,B,C and D. passed the specifications in both assay methods while F and H passed only High performance liquid chromatography and I passed only Ultra Violet Spectophotometry. However samples E. D and J failed both assay methods. Also from the percentage relative derivation and percentage coefficient of variation of High Performance Liquid Chromatography (0.0012) and Ultra Violet Spectophotometry (1.9960) it can then be concluded that High Performance Liquid Chromatography has a lesser percentage and hence a more precise and efficient method than the ultra violet.