ANALYSIS OF DIFFERENT BRANDS OF PARACETAMOL 500mg TABLETS USED IN MAIDUGURI, USING ULTRA VIOLET SPECTROPHOTOMETRIC AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC (HPLC) METHODS

Abstract

The study involves quantitative analysis of eight (8) different brands (samples) of Paracetamol 500mg tablets used in Maiduguri, using Ultra Violet Spectrophotometric and High Performance Liquid Chromatographic methods, in which the samples were dissolved in 0.1M NaOH and distilled water and their various absorbances determined at wavelength of 257nm and the HPLC method. The results obtained were compared with that of the standard. Percentage content and content in mg for each sample was calculated using the absorbances and peak areas of the samples and that of the standard, to see if it is within the specified limit by official books (90%-110% according to USP). The percentage content of the analyzed samples using HPLC method ranges from 51.04-103.84%, while using UV method it ranges from 50.19-109.1%, indicating none of the samples contains less than 50% of the active principle. It was observed that five (5) samples Neimeth, Unclu P, Palmol, Emzol, Fidson, out of the eight (8) Neimeth, Unclu P, Palmol, Emzol, Shekdol, Fidson, Nemel, Arenol, analysed meet up the USP specified limit. After the calculation of the standard deviation and coefficient of variation of the two methods used, which are 123.5 and 27.7% respectively for UV method and 82.67 and 20.4% respectively for HPLC method, it was also observed that the HPLC method is more suitable for such kind of studies than the UV method,