DEVELOPMENT AND VALIDATION OF ABSORBANCE CORRECTION METHOD FOR SIMULTANEOUS DETERMINATION OF LEVOCETIRIZINE AND AMBROXOL IN COMBINED TABLET DOSAGE FORM

Abstract

A new, simple, accurate and sensitive UV-Spectrophotometric absorbance correction method has been developed and validated for simultaneous estimation of Levocetirizine (LEV) and Ambroxol (AMB) in a combined tablet dosage form. Distill water was used as solvent. In this method, two wave lengths on Ambroxol curve were selected where it showed same absorbance, which were 261 nm and 303 nm. At 303 nm, Levocetirizine showed zero absorbance while at 261 nm it gave considerable absorbance. At 261 nm, both the drugs showed some absorbance. The absorbance of Levocetirizine was corrected at 261 nm. The method was found to be linear between the range of 4-28 $\mu$g/ml for Levocetirizine and Ambroxol. The mean percentage recovery was found in the range of 99.4 ± 1.11 % and 100.2 ± 1 % for Levocetirizine and Ambroxol respectively at three different levels of standard additions. The precision (intra-day, inter- day) of method were found within limits (RSD < 2 %). The method was validated according to ICH guidelines. Thus the proposed method was simple, precise, economic, rapid and accurate and can be successfully applied for simultaneous determination of Levocetirizine and Ambroxol in combined tablet dosage form.