SIMULTANEOUS RP-HPLC DETERMINATION OF CEFPODOXIME PROXETIL AND OFLOXACIN

Abstract

A rapid and simple high Reverse-phase performance liquid chromatography method was developed and validated for simultaneous determination of Cefpodoxime Proxetil and Ofloxacin from pharmaceutical preparation. The separation was achieved on A kromosil (technokroma) 100 C-8 (3.5$\mu$m ,15x0.46) performed using Acetonitrile and phosphate buffer as mobile phase the of pH of buffer was adjusted to 4.8 using Ortho-phosphoric acid, the ratio was 58:42 (v/v) eluents were monitored at 275 nm. The detector response was linear in the range of 100 to 300 $\mu$g, for both Cefpodoxime Proxetil and Ofloxacin. The percentage assay Cefpodoxime Proxetil and Ofloxacin was found between 99.76 and 99.31% respectively. The described method has the advantage of being rapid and easy hence it can be applied for routine quality control analysis.