STUDY OF DIURETIC ACTIVITY OF ETHANOLIC EXTRACT OF SEEDS OF HORDEUM VULGARE IN ALBINO RATS

Abstract

Background: Diuretics, which encourage the excretion of water and electrolytes, are extremely beneficial for treating a variety of illnesses, including those that cause edema, such as congestive heart failure, kidney problems, pregnancy-related toxemia, premenstrual tension, and hypertension. Objectives: To investigate, the diuretic efficacy of an ethanolic seed extract of Hordeum vulgare (EEHV) using the Lipschitz method on albino rats,. Methods: Five groups of albino rats were used to evaluate the diuretic activity of EEHV by using metabolic cages. Group I served as the normal control and received vehicle (CMC 2%).Group II received frusemide 10 mg/kg.The groups III, IV, and V were given low (100 mg/kg), medium (200 mg/kg), and high (400 mg/kg) doses of EEHV in vehicle, respectively.All the rats were immediately hydrated with saline (15 ml/kg, po) and housed in metabolic cages. At the end of the fifth hour, the total amount of urine collected was measured. Total urine volume and sodium, potassium, and chloride ion concentration in the urine, were measured and estimated. Results: The EEHV at various dose levels (100,200, and 400 mg/kg) significantly increased urine volume and increased sodium, potassium, and chloride ion elimination in urine as compared to the vehicle-treated control group. Conclusion: The amount of urine produced and the concentrations of sodium, potassium, and chloride increased significantly after a single dosage of standard frusemide (10 mg/kg) and EEHV (100, 200, and 400 mg/Kg). The higher diuretic efficacy caused by EEHV 400 mg/Kg was equivalent to that of conventional furosemide (10 mg/kg).