ANTIOXIDANT PROFILE OF CORIANDRUM SATIVUM METHANOLIC EXTRACT

Abstract

Methanolic extract of Coriandrum sativum (coriander) seeds was analyzed for the presence of various antioxidants; ascorbate, riboflavin, tocopherol, polyphenols and in vitro antioxidant potential. The extract, rich in polyphenolic compounds (18.696 ± 0.12 mg/g dry seeds) was subjected to HPLC analysis for identification and quantification of phenolics. Gallic acid (173.656$\mu$ g), caffeic acid (80.185$\mu$ g), ellagic acid (162.861 $\mu$ g), quercetin (608.903 $\mu$ g) and kaempferol (233.70 $\mu$ g)/g dry seeds were identified. Antioxidant activity of the extract was determined by various mechanisms including DPPH free radical scavenging, metal induced protein and lipid oxidation inhibition and protection of DNA against H2O2 induced damage. Coriander had excellent free radical scavenging activity with IC50 value 0.4 mg dry seed weight, whereas comparatively higher IC50 was observed with metal ion chelating assays (7.2-8.0 mg dry seed weight). The results suggest that polyphenols including gallic acid, caffeic acid, ellagic acid, quercetin and kaempferol are the principle component responsible for high antioxidant activity of methanolic extract of coriander seeds. This is the first report on detailed analysis of antioxidant composition and antioxidant properties of methanolic extract of coriander seeds.