PHYTOCHEMICALS SCREENING BY FTIR SPECTROSCOPY AND ANTIMICROBIAL ACTIVITY OF DIFFERENT SOLVENT FRACTIONS FROM MURRAYA KOENIGII L. SHOOTS

Abstract

Murraya koenigii L. (M.koenigii) is a spice herb and a medicinal plant, well known for its numerous biological activities. In the present research work, different solvent fractions from shoots of M.koenigii were analyzed for their antimicrobial activity alongwith FTIR spectroscopy. The antimicrobial activity of different M.koenigii shoot fractions was checked against different standard bacterial strains namely Escherichia coli (E.coli), Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), Salmonella typhi (S. typhi), Bacillus cereus (B. cereus), and Klebsiella pneumoniae (K. pneumoniae) alongwith antifungal activity against Candida albicans (C. albicans) respectively. Among all the tested solvent fractions (n-hexane, ethyl acetate, butanol, chloroform and water) chloroform fraction resulted maximum potent inhibitory effect against B. cereus and K. pneumoniae with maximum zones of inhibition, 15.5mm and 15.0mm alongwith inhibition zone of 12.75mm diameter against C. albicans respectively. The phytochemical analysis of M.koenigii solvent fractions showed the presence of alkanes, amines, phenols and carboxylic acid by using FTIR spectroscopy. FTIR spectroscopy revealed the presence of highest phytochemical contents in chloroform fraction among all the tested fractions that is directly related to antimicrobial activity. Only the chloroform fraction had highest antimicrobial activity with maximum phytochemicals, further investigation needed to ascertain its safety and use in drug development.