HPLC METHOD DEVELOPMENT FOR EMBELIN ESTERS DERIVATIVES

Abstract

A simple reverse phase liquid chromatographic method has been developed and subsequently validated for the derivative of the Embelin Esters substituted derivatives. The separation was carried out using a mobile phase consisting of acetic acid buffer in HPLC grade water (as mobile phase A) and methanol (as mobile phase B). An Inertsil ODS, 3.0 V (250 X 4.6 mm and 5.0 $\mu $), HPLC column was used with flow rate of 1.0 ml / min using UV-Vis detection at 260 nm of Shimadzu HPLC (model: LC-2010). The analytical method, data integrations, processes and calculations were processed by LC solution soft ware. There are ten Embelin substituted ester derivatives are taken for analysis viz., RLS 33, 34, 36, 37, 38, 40, 41, 42, 44 and 45. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of the ten derivatives interims of validation parameters viz., separation, system suitability, System Precision and linearity in a single simple HPLC run.