IN VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC ROOT EXTRACT OF DECALEPIS HAMILTONII WIGHT & ARN

Abstract

The present study was to carry out to evaluate the antioxidant potential of methanolic root extract of Decalepis hamiltonii. The methanolic root extract was subjected to total phenolic and flavonoid estimation. The level of the antioxidant potentials of methanolic root extract were determined by DPPH, ABTS, Superoxide radical, Hydroxyl radical activity. The results showed that methanolic root extract of Decalepis hamiltonii had higher level of phenol (13.05±1.01mgGAE/g)and flavonoid content (6.4±0.70mg QE/g).The methanolic root extract showed significant antioxidant activity. The percentage of inhibition for DPPH (93%), ABTS (90%), superoxide radical (80%) and hydroxyl radical (62%) which is comparable with respective standards. The results suggested that the antioxidant activity was due to higher levels of phenolic and flavonoid contents in the methanolic root extract. Further studies along with isolation and molecular mechanism of methanolic root extract of Decalepis hamiltonii may lead to significant outcome.