EXPERIMENTAL EVALUATION OF ANALGESIC EFFECT OF CLOVE OIL WITH COMPARISON OF TRAMADOL IN MICE

Abstract

Background: Pain, the most common reason for patients, is an unpleasant sensation. It is generally caused by inflammatory reaction in the tissue or tissue damage and produced emotional reaction. Clove oil is an important aromatic spice, which belongs to the Myrtaceae family, contains high number of phenolic compounds with several biological activities, including analgesic, anticonvulsant, antibacterial, antifungal, insecticidal and antioxidant properties. Eugenol is the major compound in Clove essential oil. It is used as a local antiseptic and analgesic and anesthetic. Aim: To study the analgesic activity of clove oil in comparison to tramadol in mice. Methods: This experimental study was done in central animal house, Department of Pharmacology, Gandhi medical college, study duration from 18^th august 2022 to 1^st october . Three months old Male albino mice(n=15) were divided into three groups: 1) control group treated with normal saline, 2) standard group treated with inj.Tramadol 50mg/kg(Zydus corza zydus health are Ltd)., 3) Test group treated with Inj.clove essential oil 0.1 ml/kg (Dwibhashi bapamma Ayurvedic Nilayam Pvt.Ltd.) 15 min before testing in hot plate. Analgesic effect of clove oil and Tramadol had been seen with Hot plate method as a basic method to induce their respective doses. Repeated measures of ANOVA with Post-hoc Tukey’s HSD test were used for statistical analysis. Results: Repeated measures of ANOVA with Post hoc Tukey’s HSD test showed that maximum analgesic effect in group treated by 10% Clove essential oil was significantly higher (P value<0.05) than Normal saline group and similar to Tramadol group. Conclusion: Present study results showed that Clove oil may possibly act as analgesic agent from heat with stimulates the nociceptive mechanism. Clove essential oil has analgesic action similar to Tramadol in mice using hot plate test.