PHYTOCHEMICAL SCREENING, GC-MS CHARACTERIZATION AND IN VITRO ANTIBACTERIAL ACTIVITY OF WHOLE PLANT BLEPHARIS BOERHAVIFOLIA PERS AGAINST MRSA

Abstract

Blepharis boerhavifolia (Acanthaceae) commonly known as kozhimookan has been recognized as a part of traditional medication for the treatment of various diseases. This study was carried out with an objective to investigate phytochemical compounds, antibacterial activity and GC-MS analysis of Blepharis boerhavifolia (Acanthaceae). The crude extracts of Blepharis boerhavifolia revealed the presence of several biologically active phytochemicals with the highest quantity of alkaloids, flavonoids and phenols were performed for dichloromethane, chloroform, ethyl acetate, acetone and methanol extract. The antibacterial activity was evaluated against gram positive organism and gram negative organism Enterococcus faecalis, Klebsiella pneumonia NCIM 2719, Enterobactorclocea NCIM 2164 and Clinical strain Methicillin resistant staphylococcus aureus (MRSA)by agar diffusion method. The antibacterial activity of ampicillin, ciprofloxacin and methanol extract showed maximum zone of inhibition 10 mm, 30.33 mm and 15 mm against MRSA respectively.In addition to that qualitative analysis of methanolic crude extract of Blepharis boerhavifolia was analyzed by using GC-MS showed that the presence of a ten different phytochemical compounds. The components were identified by compare their retention time and fragmentation patterns with those data stored in the National Institute of Standard and Technology (NIST) library. The reported chief constituents are Azulene derivatives, Cayophyllene, $\gamma$-Neoclovene, $\bete$-Humulene, Methanoazulene, Tetradecanoic acid and 13-Tetradecenal characterized. The GC-MS analysis revealed the presence of many bioactive compounds such as flavonoids, terpenoids, phenolics and steroids that can be postulated for antibacterial, antioxidant and anticancer activity.