IN VITRO ANTI-INFLAMMATORY AND ANTI-DIABETIC ACTIVITY OF METHANOLIC EXTRACT OF CARDANTHERA DIFFORMIS DRUCE

Abstract

In rural and backward areas of West Bengal in India, several plants were commonly used as herbal medicine for the treatment of many diseases without studying any photochemical and biological information in detail. The current study was to investigate the anti-inflammatory and antidiabetic study of methanolic extract of Cardanthera difformis. Methanolic extract of Candanthera difformis was screened for anti-inflammatory activity by denaturation of egg albumin. The methanolic extract of Candanthera difformis was subjected to in vitro inhibition of protein denaturation in various concentrations i.e. 100, 200, 400, 800, 1000 $\mu$g/ml. Methanolic extract exhibited a concentration dependent inhibition of protein (albumin) denaturation. The anti-diabetic behaviour of methanol extracts of whole plant of Cardanthera difformis using the standard optical density assay were investigated against the two very essential enzyme $\alfa$-amylase and $\alfa$-glucosidase. The intestinal digestive enzymes play a vital role in the carbohydrate digestion. Pancreatic alpha-amylase and glucosidase inhibitors offer an effective strategy to lower the levels of post prandial hyperglycaemia via control of starch breakdown. The goal of the present study is to improve in vitro evidence of potential inhibition of alpha-amylase and alpha-glucosidase enzymes by using the methanolic extract of Cardanthera difformis. The present study suggests that the crude extract of Cardanthera difformis effectively act as in vitro anti-inflammatory and in vitro anti-diabetic activity.