IN VITRO ANTIDIABETIC ACTIVITY OF AQUEOUS FRUIT EXTRACT OF Solanum torvum Sw.

Abstract

Anti-diabetic activity of aqueous fruit extract of Solanum torvum was aimed at detecting the phytochemicals and ascertains the antidiabetic activity using in vitro experimental models. In this study, physiochemical parameters were carried out and the content of phyto organic constituents such as total alkaloids, flavonoids, phenols, carbohydrates, fats, proteins and crude fiber were determined. LCMS/MS have been used for detecting the phytochemicals. Different concentrations of the extract have been screened for their anti inflammatory and antidiabetic activities. Aqueous fruit extract of S. torvum revealed the presence of (iso)pentyl dihexoside, quinic acid derivative, chlorogenic, caffeoylglucaric acid and dicaffeoylquinic acid by LC MS/ MS analysis. The extract exhibited maximum antiinflammatory activity of 23.95 % at 1000 $\mu$ g/ml in inhibition of albumin denaturation, 59.00% at 1000 $\mu$ g/ml in inhibition of protease denaturation and 64.00% at 1000 $\mu$g/ml by HRBC membrane stabilization assay, which are comparable to that of standard aspirin. The extract exhibited maximum anti diabetic activity of 72.00 % at 1000 $\mu$ g/ml in inhibition of $\alpha$-amylase activity, 63.00% at 1000 $\mu$ g/ml in inhibition of $\alpha$-glucosidase activity and 78.00% at 1000 $\mu$g/ml in glucose uptake by yeast cells, which are comparable to that of standard acarbose. From the results, the aqueous fruit extract of S. torvum might possess antidiabetic activity due to the presence of bioactive compounds.