NEW SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF PRULIFLOXACIN

Abstract

New Spectrophotometric methods (A, B and C) are described for the determination of prulifloxacin in pure form. The methods involve the formation of coloured ion pair complexes with 3-methyl-2-benzo thiazoione hydrazine (MBTH) and folin ciocalteu (FC) reagents². The complexes from MBTH with Cerric Ammonium Sulphate(CAS) and FC with sodium carbonate, MBTH with ferric chloride shows absorbance at 630nm,732nm and617nm respectively. The colours were stable for different time intervals after reaction and beers law obeyed in the concentration range of 5 to 40 µg/ml and 2.5-25$\mu $g/ml for MBTH with CAS, FC, MBTH with ferric chloride respectively¹. The molar absorptivities, standard deviation and percent recoveries were evaluated. Results of analysis were validated.