PHYSICO-CHEMICAL AND PHYTOCHEMICAL EVALUATION OF CARICA PAPAYA LINN. UNRIPE FRUITS

Abstract

Present study was performed for the development of quality standards of Carica papaya Linn. commonly known as Papita belonging to family Caricaceae is well known for its exceptional nutritional and medicinal properties. The study comprises of physico-chemical and phytochemical evaluation to confirm purity and authenticity of Carica papaya L. unripe fruit using WHO guidelines. Microscopy of the fruit showed presence thick cuticle, parenchyma, epicarp, mesocarp endocarp, calcium oxalate crystals, laticifers, etc. Successive extractive value and hot extractive value was found highest in alcoholic extract 48.34
% and 44.90 % respectively (on dry weight basis). Mean ash values (%) are 8.63 (total), 0.79 (acid insoluble ash), and 5.30 (water soluble ash) and moisture content was found to be 9.41 % and the phytochemical analysis revealed the presence of carbohydrates, terpenoids, flavonoids, phenolic compounds in different extracts of Carica papaya L. fruit. TLC fingerprinting profile of different extracts were also developed and quantification of $\bete$-carotene was also done by using nhexane:acetone (8.5:1.5) as a mobile phase at 415 nm and found to be higher in pet ether Carica papaya L. fruit extract 1.55 % w/w.