CYTOTOXICITY OF ZINC OXIDE NANOPARTICLES IN HELA CANCER CELL AND ITS EFFECT TO APOPTOSIS VIA P53 AND CASPASE PATHWAY

Abstract

Cancer is the second causing death diseases in the world. Nanomedicine emerged as a promising alternative for the treatment of cancer. HeLa is the very commonly employed model cell line for studying human cellular and molecular biology. Nanoparticles are a special institution of substances with precise capabilities and significant applications in lots of biomedical fields. Zinc oxide nanoparticles (ZNO) showed interested biocompatibility properties such selective accumulation in tumor cells. In this study, the work Zinc oxide nanoparticles are set up through Sol-Gel approach. The combination of nanoparticles distinguished through the utilization of DLS and TEM. In- vitro anticancer activity of Zinc oxide nanoparticles towards Hela cancer cell line utilizing various parameters had been studied . To exert cell growth arrest against Hela cancer cell lines had been determined by using Zinc oxide nanoparticles, the proficiency antiproliferative of ZnO NPs is because of cell dying and inducing apoptosis that affirmed by the usage of acridine orange-Ethidium bromide dual staining , Genotoxicity examine through RT- PCR analysis turned into done to detect the gene expression of Caspase-8, Caspase-9, and P53. In this study, we can suggest that ZnO NPs may well be utilized to supply a new drug restitution a chemotherapy drug and an enormous medical applications .