A RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF FEBUXOSTAT IN BULK AND PHARMACEUTICAL DOSAGE FORMS

Abstract

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Febuxostat in pharmaceutical dosage form. Isocratic elution at a flow rate of 1.0 mL min -1 was employed on a Hypersil C18 column at ambient temperature. The mobile phase consisted of acetonitrile: phosphate buffer 60:40 (v/v) and the detection wavelength was at 320 nm. Linearity was observed in concentration range of 5-30 $ \mu $g/mL. The retention time for Febuxostat was 3.4 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Febuxostat in pharmaceutical dosage forms.