BIOFUNCTIONAL PROTEIN FRACTION FROM RED ALGAE (RHODOPHYTA) EUCHEUMA SPINOSUM AS AN ANTIBACTERIAL AND ANTICANCER DRUG AGENT

Abstract

Protein fractions were isolated from red algae Eucheuma spinosum, this alga was derived from coast Takalar, Punaga Village, District Mangarabombang, Takalar. Protein fractions were macerated with Tris-HCl buffer. Ammonium sulfate was used to fractionation of proteins while determinations of protein content used Lowry method. The protein fractions were tested their activity against pathogenic bacteria. The fractions with the highest activity were combined with chloramphenicol and then was tested their activities back. The last test for protein fractions was screening toxicity; the method screening used Brine Shrimp Lethality Test (BSLT) method. The highest protein concentration on the crude extract was 96.76 mg/ml and the highest protein fraction concentration on a fraction of 20-40% (F2) was 88.42 mg/ml. The strongest protein fraction activity on a fraction 0-20% (F1) with inhibition zone is 9.09 mm to Escherichia coli bacteria and 9.56 mm to Staphylococcus aureus bacteria because the protein fraction F1 is highest so that it combined with chloramphenicol. All of a combination antibacterial activity was show an antagonistic effect against E. coli and S. aureus but did not for a combination of Protein Fraction F1: Chloramphenicol (95.35$\mu$g: 7.5$\mu$g), they shown additive effect (indifference) to S. aureus. The Protein fraction F1 from the Eucheuma spinosum algae was toxic to the Artemia salina Leach larvae; the value of LC was 7.252$\mu$g/mL. This fraction may be a potential anticancer drug agent, to prove it needs further research.

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