ISOLATION AND IDENTIFICATION OF CHEMICAL COMPOUNDS IN BUTANOL FRACTION OF POHPOHAN (PILEA TRINERVIA ROXB.) LEAVES

Abstract

Pohpohan (Pilea trinervia Roxb.), Urticaceae, is a plant known as a raw vegetable salad (lalab) in West Java. This plant has many uses and benefits, but scientific research on the content of its chemical compounds is still limited. The purpose of this study was to isolate the chemical compounds contained in n-butanol fraction of pohpohan leaves. The leaf simplicia was macerated with 70% ethanol. Ethanol extract was fractionated using n- butanol and water solvents. n-butanol fraction was purified by preparative thin layer chromatography using the n-butanol: acetic acid: water (4: 1: 5) developer, producing N isolates (green at UV 366nm). The ultraviolet spectrum Nshowed the $\lambda$ maximum wavelength at 208nm, and the infrared spectrum showed the presence of O-H groups, double bonds C = C, and C-O carbonyl. Analysis with gas chromatography-mass spectrometry (GC-MS) showed a fragmentation pattern of m / z: 180, 152, 151, 137, 95, 69, 57, 43. Based on fragmentation patterns, isolates were thought to have similar patterns with 10-methyl-9-oxabicyclo compounds [6.4 ] dodecane-1 (8) -en with 83% similarity index.