ANTIBACTERIAL POTENTIAL OF MANUKA HONEY BV 20+ JOINT AGAINST RESISTANT SALMONELLA ENTERICA SEROVAR TYPHI CLINICAL ISOLATES

Abstract

Antibiotic resistance poses a major task in managing typhoid associated with Salmonella enterica serovar Typhi (S. Typhi). Multidrug-resistant (MDR) isolates of Salmonella are prevalent in regions of Asia. Especially in areas having informal settlements with improper sanitation and clean water supply. The prime object of the study was to identify and determine the antibacterial potential of Manuka honey (MH) BV20+ joint undiluted and at different dilutions against resistant strains of S. Typhi. S. Typhi clinical isolates (n = 30) were collected from Civil Hospital Karachi, Pakistan. Antimicrobial potential of Manuka honey (MH) BV20+ joint and the sensitivity pattern of pathogen were detected by using Agar well diffusion method. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were also calculated by using micro-broth dilution technique. From thirty clinical isolates, no one showed resistance against undiluted manuka honey as well as at 4000 $\mu$L/mL dilution. No samples showed resistance against azithromycin, used as positive control. The average zone of inhibition of azithromycin against S. Typhi was 27.11 mm while that of undiluted manuka honey was 25.28 mm. MIC and MBC were found to be 4000 $\mu$L/mL. Manuka honey BV20+ joint showed good antibacterial potential against Salmonella enterica serovar Typhi indicating its significance in clinical practice as an empirical therapy. Further investigation is required to evaluate role of manuka honey as complementary and alternative medicine against Salmonella enterica serovar Typhi infection.