EVALUATION OF ANTIOXIDANT, ANTIPROLIFERATIVE AND CHEMOPROPHYLACTIC EFFECT OF APPLE CIDER VINEGAR AGAINST COLON CANCER CELL LINES

Abstract

Colon cancer is the most threatening cases in the contemporary world due to sedentary lifestyles, changes in eating habits, genetics and etiological factors. The latest statistics proves that it is expected to cause about 50,630 deaths every year. Apple Cider Vinegar (ACV) has been proved to be a very constructive and effective therapy for cancer, hyperlipidemia, hyperglycemia, urolithiasis and skin infections. Objective: The aim of the present work is mainly to evaluate the antioxidant and anticancer properties of apple cider, since there is not much literature supporting the same. Methodology: This work is a laboratory controlled prospective study conducted in our premises. ACV was prepared with ethanol and aqueous fermentation extraction method. Various established (5$\mu$g/ml, 10$\mu$g/ml, 15$\mu$g/ml, 20$\mu$g/ml, 25$\mu$g/ml and 30$\mu$g/ml) concentrations were prepared for further studies. The phytochemical analysis was performed and the DPPH assay was done for evaluating the antioxidant potency. MTT assay was performed for the evaluation of anticancer action against colon cancer cell lines (HT29) for the diluted concentrations to achieve the minimum inhibitory concentration. Results: The DPPH radical scavenging activity of ACV was found to be ranging from 46.14% to 86.75%for the various concentration ranges. The IC50 value of the extract was found to be 8$\mu$g/ml. The HT-29 cancer cell line subjected to various concentrations of apple cider vinegar, resulted in cell viability of 48.90% at 62.5$\mu$g/ml. From the graph IC50 value was calculated to be around 50-60$\mu$g/ml. Conclusion: It was concluded ACV is biologically active and effective against colon cancer.