CHARACTERIZATION of ENDOPHYTIC Bacillus toyonensis BESP21 PRODUCING ANTIBIOTICS

Abstract

This study aims to determine the character of potential antibiotic-producing endophytic bacteria isolates and to determine the type of endophytic bacteria. Research carried out includes the characterization of endophytic bacteria and biomolecular identification of bacteria. The results showed that bacterial growth was up to 22 hours, which phase was stationary at 18 hours. The optimum condition of endophytic microbes showed that bacteria at a temperature of 33⁰ C; pH 7.0; and salinity at NaCl 0.5%. Biomolecular identification using 16S rRNA method, based on phylogenetic and evolutionary distance, BESP21 isolates have a very close relationship with Bacillus toyonensis BSP21.