EFFECTS OF HYDROID Aglaophenia cupresina LAMOUROUX EXTRACT AGAINST CYTOTOXICITY ON HELA TUMOR CELLS

Abstract

Hydroid Aglaophenia cupressina Lamouroux is a marine invertebrate animal from living Phylum coelenterata attached to a sponge, rich in chemical compounds such as alkaloids, steroids, terpenoids, histamine which can be used as medicinal ingredients. The aim of this study was to find hydroid extract Aglaophenia cupressina Lamouroux which has cytotoxic properties against Hela tumor cells. The hydroid extract of Aglaophenia cupressina Lamouroux was obtained from multilevel maceration results in accordance with the polar gradient, to obtain chloroform extract, and ethyl acetate extract, and methanol extract. Cytotoxic testing for the three extracts using the MTT ({3- (4,5-dimethylthiazol-2yl) -2,5diphenyltetrazodium bromide} method assayed against Hela tumor cells with a concentration of 5 $\mu$g/mL, 10 $\mu$g/mL, 15 $\mu$g/mL, 20 $\mu$g/mL, 25 $\mu$g/mL, 30 $\mu$g/mL. From the results of this study found 3 extracts of hydroids that have IC values that differ in toxicity to Hela tumor cells. Chloroform extract has IC value = 12.79 $\mu$g/mL ethyl acetate extract with IC value = 10.52 $\mu$g/mL and methanol extract has IC value = 14.09 $\mu$g/mL, from the three extracts showed very high toxicity to cells. Although ethyl acetate extract showed the highest toxicity among the two other extracts, the IC50 value of the three extracts was $\leq $ 20 $\mu$g/mL in the category of very toxic. Based on these results, it was concluded that extracts from Aglaophenia cupressina Lamouroux hydroids had cytotoxic effects on Hela tumors so that they had the potential to be developed as basic ingredients for anticancer drugs.