PHYTOCHEMICAL ANALYSIS AND INVITRO ANTIOXIDANT POTENTIAL OF ANNONA MURICATA L.

Abstract

The traditional medical methods, especially the use of medicinal plants still play a major role in the developing countries. The history of the use of herbal medicine may be as old as the history of mankind. Many of the herbs and spices used by humans as food which yields useful medicinal compounds. Annona muricata L. leaves are traditionally used to treat diabetes. People have been consuming raw leaves of Annona muricata L. to control blood glucose levels. The acetone, methanol, hot water and successive water leaf extracts of Annona muricata L. were subjected to preliminary phytochemical screening of various plant constituents. The antioxidant potential of the Annona muricata L. leaves was established by total flavonoid content, FRAP assay, ABTS assay, Metal chelating activity, Phosphomolybdenum assay, Assay of superoxide radical scavenging activity, Free radical scavenging activity on DPPH along with the determination of total phenolic and tannin contents in the Annona muricata L. leaves extracts. This study aim is to evaluate bioactive constituents and invitro antioxidant activity of acetone, methanol, hot water and successive water extracts of Annona muricata L. leaf. Preliminary analysis revealed that all the four extracts responded positively for alkaloid, flavonoid, carbohydrate, glycosides, saponins, tannin, phytosterol and phenolics. The present study explored that Annona muricata L. shows efficient antioxidant activity and could act as safe and cost-effective with potential biological applications.