EVALUATION OF PHYTOCHEMICAL PROPERTIES AND ANTI OXIDANT POTENTIAL OF PRIVA CORDIFOLIA

Abstract

Priva cordifolia (Verbenaceae) is broadly dispersed all through India in states like Andhra Pradesh, Gujarat, Karnataka, Kerala and Tamil Nadu. The species of genus Priva are known for their rich composition of Phenolic compounds. Therefore, phytochemical properties and antioxidant potential of Priva cordifolia were evaluated in the present study. The ethanol extract of P.cordifolia [EEPC] was obtained by Soxhletion, with ethanol and was further processed for phytochemical screening, Total Phenol Content, Total Flavonoid Content and various in vitro antioxidant assays. The phytochemicals present in EEPC were Carbohydrates, Proteins, Steroids, Phenolic compounds, Flavonoids and Triterpenoids. The Total Phenolic and Flavonoid contents of EEPC were found to be 98.62 mg Gallic acid equivalents (GAE) per gram of dry extract and 49.76 mg Quercetin equivalents (QE) per gram of dry extract, respectively. In DPPH radical scavenging activity, EEPC exerted an inhibition of 71% and that of ascorbic acid was 79% at 500$\mu$g/ml. In the FRAP assay, the absorbance of EEPC clearly increased, due to the formation of the Fe2+-TPTZ complex with increasing concentration. Hydroxyl radical scavenging activity of the EEPC was 64% while ascorbic acid reached 73% at 500$\mu$g/ml. Superoxide radical scavenging activity of the EEPC was 79% while ascorbic acid reached 84% at 1000 $\mu$g/ml. Nitric oxide radical scavenging activity of the EEPC reached 54% while ascorbic acid reached 70% at 500$\mu$g/ml. The present study suggests that this plant could be of great importance for the treatment of free radical related diseases.