EVALUATION OF FREE RADICAL SCAVENGING CAPACITY AND REDUCING POWER OF POLYHERBAL FORMULATION COMPRISING OF THREE SELECTED PLANTS

Abstract

The present study was carried out to evaluate the free radical scavenging activity in the Polyherbal formulation of Kedrostis foetidissima, Mimusops elengi and Artemisia vulgaris. The free radical scavenging potential of Polyherbal formulation (PHF) was assessed for DPPH, Nitric oxide, Superoxide, Hydroxy radical and ABTS free radicals. Total Reducing power was studied by FRAP method. The Polyherbal formulation (PHF) showed effective free radical scavenging activity. IC50 value of Polyherbal formulation was compared against standard Ascorbic acid. IC50 value for DPPH is
45.16$\mu$g/ml, nitric oxide is 85. 73 $\mu$g/ml, superoxide is 48.9 $\mu$g/ml, Hydroxy radical is 113.2 $\mu$g/ml and ABTS is 93.14 $\mu$g/ml. Ferric reducing power increased with increase in concentration of Polyherbal formulation. Polyherbal formulation of Kedrostis foetidissima, Mimusops elengi and Artemisia vulgaris has potent free radical scavenging activity, therefore can be used for treatment of diseases related to oxidative stress.